The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 07, 1996
Filed:
Mar. 30, 1993
Applicant:
Inventors:
Christopher T Evans, Hertfordshire, GB;
Richard A Wisdom, Cambridge, GB;
Assignee:
Genzyme Limited, Suffolk, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435-4 ; 435 15 ; 435 26 ; 435975 ;
Abstract
Phosphoenolpyruvate carboxylase (PEPC) characterized by a specific activity of at least 40 units/mg; and/or by no more than 50% loss of activity after 60 days in 167 mM Tricine/HCl buffer, pH 8.0, and 15 mM MgCl.sub.2 ; and/or, at pH 6.5, an activity of at least 40% relative to the activity at optimum pH is disclosed. Such enzymatic activity can be obtained from serine pathway methylotrophs such as Hyphomicorbium, and is very suitable for use in measuring bicarbonate in biological samples.