The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 09, 1996
Filed:
Aug. 23, 1993
David T Berg, Beech Grove, IN (US);
Brian W Grinnell, Indianapolis, IN (US);
Eli Lilly and Company, Indianapolis, IN (US);
Abstract
The present invention is a method of using a poly-GT element with a eukaryotic promoter in the presence of an immediate-early gene product of a large DNA virus to increase transcription of DNA that encodes a useful substance. The method of the present invention requires the presence of the E1A gene product for maximum expression of the useful substance. A novel enhancer system is described comprising a cis-acting poly-GT element and a trans-acting E1A gene product, whereby the poly-GT element does not itself possess enhancer activity with certain eukaryotic promoters but rather requires the E1A gene product for enhancer activity. The present invention further comprises a number of useful expression vectors that comprise a poly-GT element with a BK enhancer in tandem with the adenovirus 2 late promoter positioned to drive expression of a variety of proteins, such as protein C, chloramphenicol acetyltransferase, tissue plasminogen activator, a modified tissue plasminogen activator, or an interferon. The present invention also comprises a method for further increasing the expression of a useful substance involving a poly-GT element, the E1A gene product, and a BK enhancer which has been placed immediately upstream (within 0 to about 300 nucleotides) of the eukaryotic promoter used in tandem with a BK enhancer.