The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 05, 1996
Filed:
Jan. 04, 1993
Norman Hill, deceased, late of Dorset, GB;
Anthony E Cass, London, GB;
Mark H Smit, Honolulu, HI (US);
Abstract
A biosensor for the detection and determination of the concentration of toxins by use of enzyme inhibition. Inhibition biosensors are affected by non-specific denaturation and substrate utilization which both result in a limited operational lifetime. These problems are mitigated by providing in an environment an enzyme which is oxidized by hydrogen peroxide the oxidized enzyme being reduced by an electron transfer agent, such as ferrocene, which is itself oxidized in the process. The electron transfer agent is capable of regeneration back to the reduced state and the extend of electron transfer regeneration gives a measure of enzyme inhibition by toxin. Electro-chemical technique allows for the generation of hydrogen peroxide from oxygen in aqueous media and the reduction of oxidized electron transfer agent. Immobilization of the enzyme to an electrode increases efficiency while potentially reducing denaturation. The biosensor can be used for the environmental determination of toxins like cyanide.