The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 20, 1996

Filed:

Dec. 10, 1993
Applicant:
Inventors:

Laurace E Townsend, Grosse Pointe Park, MI (US);

Michael J Borrelli, Troy, MI (US);

Diane E Maupin, Spring Valley, OH (US);

Assignee:

William Beaumont Hospital, Royal Oak, MI (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12M / ;
U.S. Cl.
CPC ...
43524023 ; 435240241 ; 4352891 ; 4352982 ; 4353041 ; 600 36 ; 623-1 ;
Abstract

An apparatus and method for culturing living endothelial cells is provided. The apparatus comprises a cell growth container, which in a preferred embodiment is a synthetic graft, heat crimped at both ends. The apparatus further comprises a rotation container that may contain the cell growth container and is attached to a rotation apparatus. The apparatus further comprises fixturing apparatus, which in a preferred embodiment is a pair of o-ring members, that enhances a separated condition of the cell growth container within the rotation container. The method of the present invention comprises providing a culture that includes living cells and a growth medium, introducing the culture into a cell growth container, heat crimping the cell growth container to substantially seal the cell growth container, and positioning fixturing apparatus upon the external surface of the cell growth container. This assembly is inserted into a rotation container, which is rotated by a rotation apparatus.


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