The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 13, 1996
Filed:
Mar. 30, 1993
Nestec S.A., Vevey, CH;
Abstract
A promoterless foreign gene is intergrated into the lac operon of Streptococcus thermophilus by transforming a host strain of Streptococcus thermophilus with a donor plasmid which does not replicate in the host strain. The donor plasmid includes a vector backbone and a sequence containing a foreign gene operably intergrated into at least a part of the lac operon of the host strain, in front of at least a part of the lacZ gene of the lac operon, and the sequence conserves the frame of the genomic lac operon of the host strain. Cointegrate transformants are identified in which the complete donor plasmid is integrated into the geonomic lac operon of the host strain, and an integrant transformant is then selected, the genome of which does not include the vector backbone of the donor plasmid but does include the foreign gene, which is operably integrated in front of the lacZ gene of the conserved genomic lac operon and which is stably maintained and expressed upon selective pressure on expression of the lacZ gene.