The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 1996
Filed:
Mar. 05, 1991
Aurora F DeCastro, Union, MI (US);
Surendra K Gupta, Elkhart, IN (US);
GDS Technology, Inc., Elkhart, IN (US);
Abstract
The present invention provides a kinetic or rate method for the determination of cholesterol in free or bound form with of a cholesterol oxidase enzyme obtained from Bacterial strain NRRL B-18713 which inherently has a high Michaelis and Menton, Km, of about 10-2 to 10-3 M for cholesterol and optionally, of cholesterol esterase. The method can measure oxygen consumption, hydrogen peroxide or cholestenone formed. The present invention also provides a test composition for the kinetic determination of cholesterol, comprising cholesterol oxidase with an inherently high Km of about 10-2 to 10-3 M for cholesterol and optionally cholesterol esterase and a system for the determination of hydrogen peroxide, cholestenone or oxygen consumption.