The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 30, 1996
Filed:
Jan. 05, 1993
Applicant:
Inventors:
David H Gelfand, Oakland, CA (US);
Pamela M Holland, Seattle, WA (US);
Randall K Saiki, Richmond, CA (US);
Robert M Watson, Berkeley, CA (US);
Assignee:
Hoffmann-La Roche Inc., Nutley, NJ (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 435810 ; 436501 ; 536 221 ; 536 231 ; 536 241 ; 536 243 ; 536 2431 ; 536 2432 ; 536 2433 ; 935 77 ; 935 78 ; 935 88 ;
Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Published as:
CA2088683A1; WO9202638A1; AU8651091A; US5210015A; EP0543942A1; JPH06500021A; US5487972A; AU666837B2; US5804375A; JP2825976B2; EP0919565A2; CA2088683C; EP0919565A3; EP0543942B1; ATE198912T1; DE69132521D1; US6214979B1; ES2155058T3; DK0543942T3; DE69132521T2; EP1302549A2; EP0919565B1; ATE252110T1; DE69133329D1; EP1302549A3; DK0919565T3; ES2209033T3; DE69133329T2; US2005255499A1; EP0543942B2; US7141377B2; DK0543942T4; ES2155058T5; EP1302549B1; DE69132521T3; ATE365812T1; DE69133574D1; DK1302549T3; ES2289190T3; DE69133574T2; US2008171315A1; US7445900B2; EP0919565B2; DK0919565T4; ES2209033T5; DE69133329T3;