The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 09, 1996
Filed:
Feb. 25, 1994
Pramod K Gupta, Federal Way, WA (US);
Gerald S Pullman, Alpharetta, GA (US);
Weyerhaeuser Company, Tacoma, WA (US);
Abstract
The invention is a method for reproducing Douglas-fir by somatic embryogenesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage embryos. These are multiplied in a second culture having reduced growth hormones where the early stage embryos grow in size and vigor to advanced early stage embryos. The embryos may then be transferred directly to a cotyledonary embryo development culture containing an adsorbent such as activated charcoal. Preferably, they are first singulated by shaking in two or more liquid subcultures containing the adsorbent prior to placing them on the development medium. After several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth.