The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 02, 1996
Filed:
Feb. 16, 1994
Antonio T Francavilla, Pittsburgh, PA (US);
Michio Hagiya, Shiga, JP;
Thomas E Starzl, Pittsburgh, PA (US);
Toyobo Co., Ltd., Osaka, JP;
University of Pittsburgh, Pittsburgh, PA (US);
Abstract
A full-length cDNA clone has been isolated encoding a purified augmenter of liver regeneration (ALR) polypeptide prepared from the cytosol of livers from weanling rats. The 1.2 kb cDNA includes a 299 bp 5'-untranslated region, a 375 bp coding region and a 551 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081 which is consistent with the size estimated by SDS-PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS-PAGE under non-reducing conditions is about 30,000, apparently with a homodimer structure. The recombinant ALR produced by expression of the cDNA in cultured cells was tested in vivo in the canine Eck's fistula model and found to have a potency equivalent to the purified native ALR. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.