The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 28, 1995

Filed:

Apr. 12, 1994
Applicant:
Inventors:

Edward F Mussi, Hewitt, NJ (US);

Harry E Gray, Bloomingdale, NJ (US);

Assignee:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M / ;
U.S. Cl.
CPC ...
4352971 ; 4352975 ; 4353031 ; 4353052 ; 4352873 ; 4352878 ; 4352884 ;
Abstract

A removable cell culture insert support device is provided which allows the initial development of cell cultures on the bottom membrane surface of an inverted cell culture insert. Following successful cell culture development, the support device of the invention is discarded and the conventional insert is reoriented and then suspended in a conventional multi-well plate with the initially developed cells on the bottom surface of the insert membrane. The upper surface of the membrane is then available for developing a second cell type for transmembrane interaction as required. The removable support includes a cup-shaped chamber with a surrounding skirt to accommodate the cell insert of interest, with the removable support having appropriate cooperating attaching surfaces. A double-sided adhesive may be used to temporarily join the two parts together.


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