The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 1995
Filed:
Aug. 10, 1994
Applicant:
Inventors:
Tatsuya Ohwaki, Ohimachi, JP;
Hiroshi Sakai, Ohimachi, JP;
Assignee:
Nisshin Flour Milling Co., Ltd., Tokyo, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435212 ; 435219 ; 435226 ;
Abstract
A human endothelin converting enzyme (ECE) has been isolated from blood and placenta. The enzyme has the activity of converting big endothelin-1, -2, or -3 to endothelin-1, -2, or -3 respectively. The enzyme has a mass of about 5.4.times.10.sup.5 by gel filtration, a pH optimum of 6.5 to 7.5, and a hydrated density of less than 1.210. The enzyme is inhibited by EDTA, phosphoramidon, thiorphan, chymostatin and phenylmethylsulfonylfluoride (PMSF). The enzyme is precipitated by 0.65% dextran sulfate or 0.2M manganese chloride. The enzyme can be inactivated by oxidation by copper ion.