The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 14, 1995
Filed:
Mar. 08, 1993
Applicant:
Inventors:
Glenn Dorin, San Rafael, CA (US);
David R Gray, El Cerrito, CA (US);
Byeong S Chang, Orinda, CA (US);
Cynthia A Cowgill, Berkeley, CA (US);
Robert J Milley, Berkeley, CA (US);
Assignee:
Chiron Therapeutics, Emeryville, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K / ; C07K / ; C07K / ; C12P / ;
U.S. Cl.
CPC ...
530351 ; 530412 ; 530414 ; 530427 ; 435 711 ; 435 712 ; 435 695 ; 424 851 ;
Abstract
A process is described for producing M-CSF from bacteria. It includes: fermentation of bacteria containing M-CSF DNA; harvest of the fractions that contain the M-CSF protein (refractile bodies); primary recovery of the protein; solubilization and denaturation of refractile bodies; M-CSF refolding; purification by column chromatography and other methods; and formulation of the properly refolded M-CSF. This method is advantageous over prior methods in terms of yield and purity.