The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 17, 1995
Filed:
May. 02, 1994
Applicant:
Inventors:
Christopher D Wolin, Milwaukee, WI (US);
Phillip P Franciskovich, Brown Deer, WI (US);
Assignee:
Pharmacia P-L Biochemicals Inc., Milwaukee, WI (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12Q / ; C07H / ; C07H / ;
U.S. Cl.
CPC ...
536 2542 ; 536 254 ; 536 2541 ; 435-6 ; 4351723 ; 435810 ; 935-4 ; 935 21 ;
Abstract
A method kit for purification of m-RNA from a cell are disclosed. Guanidine containing moieties, at high molarity, are use to quickly lyse the cell. They also act to inhibit RNase activity. Without the need for isolation of total RNA, the lysate can then be directly purified from the lysate using oligo dT (or U) by reducing the guanidine concentration via a dilution step.