The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 1995
Filed:
Aug. 12, 1993
David Naveh, Leiden, NL;
John C Tang, Livingston, NJ (US);
Schering Corporation, Kenilworth, NJ (US);
Abstract
An ion-exchange chromatography technique for purifying crude proteins containing closely related impurities, wherein the isoelectric points of the desired protein and the impurities are determined, is disclosed. In performing this technique, the pH for the ion-exchange chromatography is adjusted to a point between the ranges of isoelectric points of the protein fractions to be separated whereby, upon contacting the crude protein mixture at such pH with an ion exchange resin, the proteins in the first and second fractions are oppositely charged and only one of the fractions binds to the ion exchange resin. The application of the technique to the purification of GM-CSF using cation exchange resin is also disclosed.