The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 08, 1995

Filed:

Mar. 14, 1994
Applicant:
Inventors:

Nobuhiko Yamashita, Takatsuki, JP;

Junichi Tohyama, Ohtsu, JP;

Chiwa Kataoka, Kyoto, JP;

Kazunobu Miura, Nagaokakyo, JP;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C07H / ;
U.S. Cl.
CPC ...
435 9153 ; 435 913 ; 435 915 ; 536124 ;
Abstract

A method for preparing aminoacyl- and misaminoacyl-tRNAs utilizing a specific aminoacyl-tRNA hydrolase is provided. According to the present method, aminoacyl-tRNAs consisting of a tRNA and a corresponding amino acid, or misaminoacyl-tRNAs consisting of a tRNA and a non-corresponding amino acid or an amino acid derivative, can be prepared. In a specific embodiment, L-tyrosyl-tRNA.sup.Tyr is prepared using aminoacyl-tRNA hydrolase from Fusarium culmorum or Artemia salina. In another embodiment a misacylated tRNA, L-phenylalanyl-tRNA.sup.Tyr, is prepared using the aminoacyl-tRNA hydrolase.


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