The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 11, 1995
Filed:
Jun. 01, 1993
Cesira Galeotti, Siena, IT;
Emanuela Palla, Montecatini Terme, IT;
Giovanni Raugei, Florence, IT;
Giuliano Bensi, Florence, IT;
Maria L Melli, Siena, IT;
Sclavo, S.p.A., Siena, IT;
Abstract
The invention provides an expression and secretion vector in yeasts which is useful for preparing heterologous proteins, comprising a synthetic oligonucleotide which directs the secretion of the heterologous protein wherein the synthetic oligonucleotide is positioned between the inducible hybrid promoter GAL-CYC and a multiple-site polylinker followed by the signals of transcription termination recognized by the RNA polymerase of the yeasts. The invention includes a hybrid plasmid obtained by cloning in one of the restriction sites of the polylinker of the vector, the DNA sequence which codes for a heterologous protein. A process is also described for the preparation of heterologous proteins which comprises cultivating in a suitable culture medium a yeast transformed with the hybrid plasmid and recovering from the culture medium the resulting heterologous proteins. The recovered proteins include hormones, lymphokines, viral antigens or immunogens useful in the therapeutical and diagnostic field.