The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 11, 1995
Filed:
Mar. 30, 1993
Carl W Fuller, Cleveland Heights, OH (US);
United States Biochemical Corporation, Cleveland, OH (US);
Abstract
Method for performing a cycled primer extension reaction by contacting a template DNA with a primer in the presence of sufficient glycerol or ethylene glycol to lower the melting temperature of template DNA and primer hybrids below 70.degree. C. and a DNA polymerase under conditions in which the DNA polymerase can cause primer extensions and is stable to the temperature at which the reaction mixture is heated to denature the primer extension product from the template nucleic acid; and a kit suitable for use in cycle primer extension reaction including the necessary primers, buffers and enzymes required for the procedure, and glycerol.