The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 20, 1995
Filed:
Sep. 08, 1993
Applicant:
Inventors:
Robert B Wallace, Greenbrae, CA (US);
Franklin R Witney, Novato, CA (US);
Assignee:
Bio-Rad Laboratories, Inc., Hercules, CA (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ;
U.S. Cl.
CPC ...
435 912 ; 435 914 ; 435 9151 ; 43525233 ;
Abstract
This invention relates to a method of cloning DNA produced by primer extension including PCR amplified, reverse transcriptase-generated or primer extended synthetic DNA. Specifically, it relates to a method in which alkane diol residue containing oligonucleotide primers are incorporated into DNA by primer extension followed by direct cloning of the target DNA. Following transformation, the host excises the alkane diol residue with its endogenous DNA repair machinery.