The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 23, 1995
Filed:
Dec. 06, 1991
Edward Nudelman, Seattle, WA (US);
Anil Singhal, Seattle, WA (US);
Henrik Clausen, Seattle, WA (US);
Sen-itiroh Hakomori, Mercer Island, WA (US);
Kazuo Muroi, Minamikawachi, JP;
Toshio Suda, Minamikawachi, JP;
Hisao Nojiri, Seattle, WA (US);
The Biomembrane Institute, Seattle, WA (US);
Jichi Medical School, Tochigi, JP;
Abstract
A method of producing monoclonal antibodies that bind to tumor-associated gangliosides, the method comprising: (1) immunizing a host with tumor cells; (2) boosting the host with a suspension comprising a mixture of tumor cell membrane and at least one purified lactonized tumor-associated ganglioside; (3) boosting the host with an immunogen comprising a lactone of a tumor associated ganglioside adsorbed on or incorporated into a carrier; (4) fusing immunized cells from the host with myeloma cells to form hybridoma cells; (5) selecting hybridoma cells that produce antibody that binds to the ganglioside of step (3); (6) culturing the selected hybridoma cells; and (7) recovering the antibody.