The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 16, 1995

Filed:

Oct. 21, 1993
Applicant:
Inventors:

Peter Zaun, Libertyville, IL (US);

Stanley R Bouma, Grayslake, IL (US);

Julian Gordon, Lake Bluff, IL (US);

John J Kotlarik, Vernon Hills, IL (US);

Assignee:

Abbott Laboratories, Abbott, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12M / ;
U.S. Cl.
CPC ...
422 64 ; 422 67 ; 422 8205 ; 422104 ; 422109 ; 422119 ; 435290 ; 435291 ; 435310 ; 435316 ; 219385 ; 219390 ; 219521 ; 219535 ;
Abstract

Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target.

Published as:

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