The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 09, 1995

Filed:

Jul. 18, 1990
Applicant:
Inventors:

Craig R Malloy, Dallas, TX (US);

F Mark Jeffrey, Dallas, TX (US);

A Dean Sherry, Dallas, TX (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 35 ; 435-4 ; 435 14 ; 435 29 ; 435 30 ; 436 56 ; 436 57 ; 436 63 ; 436173 ; 424-93 ; 424-935 ;
Abstract

The present invention relates to a method of measuring the contribution of one or more exogenously administered .sup.13 C-labeled substrates to acetyl-CoA. The measurement can be made in a tissue or cell using .sup.13 C NMR without the constraint of metabolic or isotopic steady-state. Furthermore, the method permits the determination even when spectral lines are broad due to B.sub.0 inhomogeneity, thereby opening the way for substrate utilization studies in vivo. The method does not require many of the simplifying assumptions involved in .sup.11 C or .sup.14 C methods, and, since a stable isotope, .sup.13 C, is used a wide variety of compounds with complex labeling patterns may be synthesized and studied.


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