The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 18, 1995
Filed:
Feb. 09, 1994
Lawrence Kass, Hinckley, OH (US);
Cytocolor Inc., Hinckley, OH (US);
Abstract
Peripheral blood lymphocytes displayed a plurality of sizes and colors when exposed first to a fixative solution of C.I. basic blue 141, then to an aqueous alkaline solution of the same dye and subsequently treated with a neutral HEPES buffer solution containing small but effective amounts of various metal compounds. As confirmed with purified lymphocyte subpopulations obtained with a cell sorter, T helper cells (CD4) were small and their nuclei and cytoplasm stained deep blue. T cytotoxic/suppressor cells (CDS) were larger than T helper cells, their nuclei stained pale green or blue green and their cytoplasm contained a cluster of magenta colored granules. Used in the process described, basic blue 141 provides means of identifying and differentiating CD4 and CD8 cells under the ordinary light microscope without using monoclonal antibodies or fluorescence.