The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 07, 1995

Filed:

Apr. 24, 1992
Applicant:
Inventors:

Toyoji Sawayanagi, Tokyo, JP;

Mina Saito, Tokyo, JP;

Toshi Tsuzuki, Tokyo, JP;

Yoshio Fujiwara, Tokyo, JP;

Yoshitaka Noguchi, Tokyo, JP;

Assignee:

Showa Denko K.K., Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C11D / ;
U.S. Cl.
CPC ...
435221 ; 435219 ; 435220 ; 25217412 ;
Abstract

An alkaline protease, method for producing the same and detergents containing the protease as an aid are disclosed. The alkaline protease is obtained by cultivating in a medium the microorganism SD523 FERM BP-3774 or artificial mutants or genetically engineered variants thereof which have the ability of producing the alkaline protease. The alkaline protease has: (1) an optimum pH of from about 11 to 11.5 as measured after reaction at 30.degree. C. for 10 minutes using casein as a substrate and an optimum temperature of about 60.degree. C. as measured after reaction at pH 10 using casein as a substrate; (2) a half inactivation temperature of about 55.degree. C. as measured after reaction at pH 10 for 10 minutes; and (3) a molecular weight of 29,000.+-.2,000 as measured by SDS polyacrylamide gel electrophoresis. The enzyme has improved storage ability in liquid detergents as compared with known alkaline proteases produced by known microorganisms belonging to the genus Bacillus firmus.


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