The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 24, 1995

Filed:

Jun. 09, 1992
Applicant:
Inventor:

Fred T Oakes, Rochester, NY (US);

Assignee:

Eastman Kodak Company, Rochester, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C07H / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 435 912 ; 536 2433 ; 935 77 ; 935 78 ;
Abstract

Amplification, replication or detection of a predetermined target nucleic acid can be carried out using a unique primer composition. This composition comprises and aqueous mixture of a first oligonucleotide primer which is substantially complementary to a first nucleic acid sequence of the target, but which is suspected of having one or more mismatches with the target at or near its 3' end. Also included in the composition is one or more additional primers which are complementary to a nucleic acid sequence of the target. This sequence is either: (i) inclusive of only a portion of the first nucleic acid sequence, (ii) immediately adjacent to the first nucleic acid sequence, or (iii) removed from the first sequence by one or more bases, but which additional primer is capable of forming a primer extension product complementary to the first sequence. These composition components can be supplied as part of a diagnostic test kit which can include other regents if desired.


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