The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 17, 1995

Filed:

Sep. 15, 1992
Applicant:
Inventors:

Christopher N Bush, Richmond, NH (US);

Charlene A Audette, Keene, NH (US);

Michael A Harvey, Spofford, NH (US);

Assignee:

Schleicher & Schuell, Inc., Keene, NH (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12Q / ; C12Q / ; G01N / ;
U.S. Cl.
CPC ...
435 41 ; 435-4 ; 435-71 ; 435-79 ; 435 14 ; 435 18 ; 435 19 ; 435-791 ; 435 21 ; 435 25 ; 435 28 ; 436169 ; 436170 ; 436807 ;
Abstract

The invention provides a method for supplying an enzyme substrate to a membrane-based, enzyme-linked reaction, comprising providing an open pore, high liquid retention capacity material impregnated with a predetermined amount of a substrate for the enzyme; and contacting the material with a membrane containing the enzyme-linked reaction under conditions which permit diffusion of the enzyme substrate to sites on the membrane containing the enzyme linked reaction. The invention further provides devices for membrane-based enzyme-linked reactions comprising a porous material impregnated with a predetermined amount of a substrate for the enzyme, wherein the porous material is paper, glass fiber paper, stainless steel mesh, woven and nonwoven nylon, polyester, polyethylene, polypropylene, or fluorocarbon polymers having a porosity ranging from about 5 microns to 5000 microns; and a microporous membrane of cellulose or a cellulose derivative, nylon, polysulfone, polypropylene, PTFE, or PVDF having a porosity ranging from about 0.05 microns to about 12 microns.


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