The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 10, 1995
Filed:
Jun. 07, 1991
Joseph E Celebuski, Gurnee, IL (US);
Roger A Jones, Glenside, PA (US);
Abbott Laboratories, Abbott Park, IL (US);
Abstract
Oligonucleotide phosphorylating reagents wherein the silyl moiety has three bulky substituents, such as phenyl or t-butyl, and the silyl moiety is attached to the carbon of an ethanol group whose hydroxyl is an activated phosphate group. The preferred compound is 2-triphenylsilylethyl-2-cyanoethyl-N,N diisopropylaminophosphoramidite. The method of using this reagent to phosphorylate the 5'-OH of nucleosides and oligonucleotides is also disclosed. Nucleosides and nucleotides whose 5'-OH has been phosphorylated by the above reagent are also disclosed. The phosphorylated intermediate bearing the silyl group may be separated from failure product on the basis of bulky substituents on the silyl protecting group, which can be later removed by fluoride ion.