The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 15, 1994

Filed:

Jan. 18, 1991
Applicant:
Inventor:

Jean De Lafonteyne, Oudenaarde, BE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
4351723 ; 536 237 ; 536 241 ;
Abstract

The invention relates to a process for transforming cells and to a recombinant DNA molecule for use therein. Said recombinant DNA molecule is a multifunctional linker comprising a DNA sequence which is homologous to a part of the receptor genome, an operator sequence and either a sequence which allows replication in a microorganism or a sequence which allows for integrative recombination in a vector. Preferably, said multifunctional linker also contains a gene whose expression can be detected in a microorganism, a gene whose expression can be detected in the receptor, a sequence which promotes recombination, and/or a hairpin structure at one or both ends. The transformation process uses donor DNA coupled to the multifunctional linker and allows for early success followed by further characterization of the donor DNA and its phenotypic impact.


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