The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 18, 1994
Filed:
Apr. 23, 1993
Applicant:
Inventors:
Leif Kongerslev, Birkerod, DK;
John Pedersen, Kokkedal, DK;
Assignee:
Novo Nordisk A/S, Bagsvaerd, DK;
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C07K / ; A61K / ;
U.S. Cl.
CPC ...
435 696 ; 435 691 ; 4352402 ; 4352403 ; 43524031 ; 4353201 ; 530359 ; 530383 ;
Abstract
In a method for effecting an increased expression in serum-free medium of recombinant proteins in a host cell being able to express said protein said host is cultured in a serum-free medium comprising an egg yolk fraction being free of lipoprotein and lipids so as to express said protein. Also the use of an egg yolk fraction being free of lipoprotein and lipids for increasing the expression of recombinant proteins in a host cell being able to express said protein in a serum-free cell growth medium is disclosed.