The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 20, 1994
Filed:
Dec. 16, 1992
Edmund Lin, Boston, MA (US);
Bryan L Ray, Burlington, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Abstract
Disclosed is a method for isolating a mutant cell that secretes a desired compound at a level greater than that secreted by a starter cell from which the mutant cell is derived. The method includes contacting a plurality of auxotrophic feeder cells and auxotrophic starter cells together in the solid growth medium. The starter cells produce the desired compound required by the feeder cells and the feeder cells produce a metabolite required by the starter cells. These cells are cultured to produce a plurality of primary colonies, the largest of which include at least one of the mutant cells which overproduces the desired compound.