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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 5340731 B1

PDFFull Text
Date of Patent:
Aug. 23, 1994

Filed:

Apr. 08, 1992

Method of preparing a b-1,4 glycan matrix containing a bound fusion

Applicant:
Inventors:

Douglas G Kilburn, Vancouver, CA;

Robert C Miller, North Vancouver, CA;

Neil Gilkes, Vancouver, CA;

R Antony Warren, Vancouver, CA;

Assignee:

University of British Columbia, Vancouver, CA;

Attorney:

Rae-Venter; Barbara

Primary Examiner:

David M Naff

Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
435179 ; 435 691 ; 435 6952 ; 435 697 ; 435 711 ; 4351723 ; 435177 ; 435195 ; 435200 ; 435209 ; 435803 ; 436530 ; 530808 ; 530814 ; 935 11 ; 935 14 ;
Abstract

A fusion protein that can function as a removable label is prepared containing a polypetide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with a .beta.-1,4 glycan matrix such as cellulose, the substrate binding region binds to the matrix to immobilize the polypeptide. The polypetide or fusion protein can be removed from the matrix with a protease capable of cleaving a specific protease cleavage site, or with a solution having a low ionic strength or a high pH. The fusion protein can be prepared by recombinant DNA technology.

Find Patent Forward Citations

Kilburn, Douglas. (1994). Method of preparing a b-1,4 glycan matrix containing a bound fusion (U.S. Patent No. 5340731). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/5340731

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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