The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 09, 1994
Filed:
Jan. 22, 1993
Applicant:
Inventors:
Assignee:
Boehringer Mannheim GmbH, Mannheim-Waldhof, DE;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ;
U.S. Cl.
CPC ...
435 691 ; 4351723 ; 435215 ; 435226 ; 43525233 ; 4353201 ; 536 241 ; 935 44 ;
Abstract
An expression enhancer has a DNA sequence which is capable of forming a t-RNA clover-leaf structure after transcription and hybridizes in that region of the DNA which, after transcription, forms the anticodon loop in the clover-leaf structure with an oligonucleotide with the sequence 5'-GACTTAGAAGGTCGTT-3' or its complementary sequence (5'-AACGACCTTCTAAGTC-3'). It can be used to increase the yield in the expression of a recombinant gene by transformation of suitable host cells with an expression vector containing the recombinant gene, whereby it is likewise introduced into the host cells in a form capable of expression and expressed.