The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 21, 1994

Filed:

Dec. 21, 1992
Applicant:
Inventors:

Anthony D Genovesi, Sycamore, IL (US);

Richard A Yingling, DeKalb, IL (US);

Assignee:

DeKalb Plant Genetics, DeKalb, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01H / ;
U.S. Cl.
CPC ...
4352405 ; 4352404 ; 43524045 ; 43524048 ;
Abstract

This invention relates to the regeneration of fertile monocotyledonous plants from anther or microspore cultures. Fertile corn plants have been regenerated from in vitro cultures by the methods disclosed herein. The invention relates to synergistic effects of pretreatment with a combination of stresses, for example, a carbon structure in the preculture medium capable of inducing embryogenesis such as mannitol at cold temperatures, for example, 10.degree. C. A solid support system has been developed to transfer isolated microspores through a series of subcultures containing different media. The composition of the media are aspects of the invention. Chromosome doubling methods for haploid anther and isolated microspore cultures are presented. This invention also relates to the plants regenerated from microspore or anther cultures, and to their seeds and progeny.


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