The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 10, 1994

Filed:

Jun. 17, 1992
Applicant:
Inventors:

Milos V Novotny, Bloomington, IN (US);

Osamu Shirota, Yokohama, JP;

Donald Wiesler, Bloomington, IN (US);

Assignee:

Indiana University Foundation, Bloomington, IN (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ; C07C / ; C07C / ;
U.S. Cl.
CPC ...
436128 ; 436 93 ; 436 94 ; 436127 ; 436129 ; 436161 ; 436162 ; 436172 ; 549438 ; 549439 ; 560 21 ; 560 22 ; 560 45 ; 560 48 ; 562435 ; 562437 ; 562452 ; 562458 ;
Abstract

Compounds containing .alpha.-discarbonyl groups are rendered capable of fluorescent emission by conjugation with carboxylic acid-terminated ortho-diaminoaryl reagents. The carboxylic acid groups serve as ionizable moieties which facilitate the conjugation reaction, and are retained in the conjugation product to enable, or enhance the ability of, the conjugated compounds to be separated in chromatographic and electromigration separation processes, notably electrophoresis. Ortho-dinitro derivatives of the reagents are also disclosed, these derivatives offering the advantage of stability as well as the ability to be used directly in the reaction in the presence of a reducing agent.


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