The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 19, 1994
Filed:
Sep. 03, 1991
Edgar C Lawson, Labadie, MO (US);
James D Weiss, High Ridge, MO (US);
Cynthia L Hemenway, St. Louis, MO (US);
Nilgun E Tumer, Chesterfield, MO (US);
Monsanto Company, St. Louis, MO (US);
Abstract
A DNA sequence encoding a potato leafroll virus coat protein having at least one internal translation initiation codon in a different reading frame than the native PLRV coat protein DNA sequence altered to a non-initiator codon is provided. Two translation initiation sites at the start of a 17kd open reading frame in a different reading frame than the native PLRV coat protein DNA sequence are preferably altered A stronger stop codon is also provided in the modified PLRV DNA sequence. The modified DNA sequence having the internal translation initiation codons at the start of the 17kd open reading frame altered to non-initiator codons can be used in a gene to transform plants of the Solanaceae family to obtain transgenic plants resistant to PLRV. A synthetic modified potato leafroll virus DNA sequence is also provided which has the changes in the translation initiation sites and is further made to be more 'plant-like'. Multiple copies of the modified or synthetic modified PLRV coat protein DNA sequence driven by different promoters have also been transformed into potato with advantageous results. The modified potato leafroll virus DNA sequences of this invention are particularly useful in obtaining transgenic potato plants resistant to infection by potato leafroll virus