The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 19, 1994
Filed:
Mar. 14, 1990
Applicant:
Inventors:
Kouji Takeuchi, Nagareyama, JP;
Hideo Kawaguchi, Hachioji, JP;
Tadashi Ishibashi, Kodaira, JP;
Norio Shimizu, Kokubunji, JP;
Assignee:
Hitachi, Ltd., Tokyo, JP;
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
43524025 ; 4352401 ; 4352402 ;
Abstract
Stable, long-term cultures of animal cells are accomplished by calculating the ratio of lactic acid formed by the cells to the glucose consumed by the cells (the L/G ratio) and controlling the cultivation conditions to maintain the L/G ratio between the limits of from greater than 0 to 0.6. When the ratio is outside the desired range, the dissolved oxygen concentration in the medium is increased and/or the glucose concentration in the medium is decreased.