The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 12, 1994

Filed:

Jun. 05, 1992
Applicant:
Inventors:

Shoichi Tokutake, Noda, JP;

Tadashi Tomikura, Noda, JP;

Kazuo Kotani, Tokyo, JP;

Kazunori Saito, Tokyo, JP;

Kohichiro Tobe, Noda, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 22 ; 536-41 ; 536 173 ; 536 175 ; 536 177 ; 536 184 ; 536 187 ; 53612313 ; 435 96 ; 435 98 ; 435 99 ;
Abstract

The present invention provides a maltooligoside derivative represented by the formula: ##STR1## wherein n denotes an integer 3-5, R represents an aromatic chromophoric group, X represents a group >CHCH.sub.2 N.sub.3 or >C.dbd.CH.sub.2, and Y represents a hydrogen atom, a substituted or unsubstituted hydrocarbon group or an alkyl- or arylsulfonyl group, a reagent for determining .alpha.-amylase activity which comprises said maltooligoside derivative as an effective ingredient, and a process for determining .alpha.-amylase activity, characterized in that said maltooligoside derivative and coupled enzymes are added to an .alpha.-amylase containing sample to conduct an enzymatic reaction and a liberated aromatic chromophoric compound is quantitatively determined. The compound of the present invention is very useful as a substrate for determining .alpha.-amylase activity, and .alpha.-amylase activity can be easily measured accurately in a short time by using the reagent without affection of the other ingredients contained in the sample.


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