The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 07, 1993
Filed:
Dec. 14, 1990
Applicant:
Inventor:
Richard G Buckholz, Encinitas, CA (US);
Assignee:
Phillips Petroleum Company, Bartlesville, OK (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
435 691 ; 435 699 ; 435196 ; 435215 ; 43525423 ; 435 19 ; 4353201 ; 435938 ; 536 2374 ; 536 241 ; 935 10 ; 935 28 ; 935 37 ; 935 69 ;
Abstract
The present invention discloses the Pichia pastoris acid phospbatase gene, which includes the 5' regulatory region, signal sequence, structural gene, and 3' transcription termination sequence. Also disclosed are methods of using these fragments, which include but are not limited to the secretion of proteins from cells and the regulation of the transcription of DNA. DNA vectors containing the acid phospbatase gene or fragments thereof and hosts transformed with these vectors are also disclosed. Additionally, integrative vectors which direct integration at the Pichia pastoris PHO1 locus and a method of identifying these disruptants is disclosed.