The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 16, 1993

Filed:

Nov. 13, 1990
Applicant:
Inventors:

Ramon A Evangelista, Scarborough, CA;

Eva F Templeton, Willowdale, CA;

Alfred Pollak, Toronto, CA;

Assignee:

Kronem Systems, Inc., Ontario, CA;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12G / ;
U.S. Cl.
CPC ...
435-6 ; 435 21 ; 435 25 ; 435 28 ; 435968 ; 435-74 ; 435-791 ; 436 56 ; 436501 ; 436512 ; 436546 ; 436800 ; 536 231 ; 935 76 ; 935 77 ; 935 98 ; 935 81 ;
Abstract

A method and compounds useful for this method are described for enzyme-amplified signal detection in analytical assays requiring extremely high detection sensitivity which uses a substrate capable of being transformed by an enzyme from a compound which does not form a luminescent lanthanide chelate into a product which forms a luminescent lanthanide chelate. The method in which a substrate not capable of forming a highly luminescent lanthanide chelate is enzymatically altered to produce a product which forms a highly luminescent lanthanide chelate and hence is particularly useful in time-resolved luminescence analysis as required in many different heterogeneous or homogeneous assay formats is described herein.


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