The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 12, 1993

Filed:

Feb. 22, 1991
Applicant:
Inventors:

Immaculada Margarit y Ros, San Donato Mil., IT;

Susanna Campagnoli, Codogno, IT;

Roberto Gianna, Rome, IT;

Salvatore Toma, Milan, IT;

Guido Grandi, Segrate, IT;

Assignee:

Eniricerche S.p.A., Milan, IT;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435222 ; 435221 ; 435 691 ; 43525231 ; 4353201 ; 536 232 ;
Abstract

The mutants of neutral protease (NP) described can retain their enzymatic activities at temperatures at which wild-type neutral protease becomes inactive, the mutants being characterised in that the Gly 189 and/or Gly 147 residues of the aminoacid sequence of NP are replaced by different residues selected from the natural aminoacids. Mutagenised genes of neutral protease which code for the mutants, recombinant plasmids containing the genes, and strains of Bacillus transformed by the plasmids are also described. The mutants of neutral protease having the characteristics described above are particularly useful in the food sector.


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