The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 05, 1993
Filed:
Sep. 20, 1991
Applicant:
Inventors:
James F Jolly, Glendale, WI (US);
Jack G Chirikjian, Potomac, MD (US);
Caecilia J Huang, New Berlin, WI (US);
Assignee:
Pharmacia P-L Biochemicals Inc., Milwaukee, WI (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435196 ; 435 18 ; 435 19 ; 435178 ; 435183 ; 435187 ; 435188 ; 435195 ;
Abstract
A room temperature stable restriction enzyme in a glassified carbohydrate stabilizer is disclosed. A restriction enzyme reaction buffer containing Mg.sup.+2 is dispersed in the stabilizer. One cleaves DNA merely by adding water and DNA to the composition. The composition can reduce glycerol-caused star activity by avoiding the need for glycerol. The composition also reduces star activity caused by high concentrations of enzymes. Another method is provided of comparing cleaved DNA samples in connection with forensic and paternity testing applications.