The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 31, 1993
Filed:
Apr. 24, 1991
William R Heineman, Cincinnati, OH (US);
Hua-Tang Tang, Cincinnati, OH (US);
H Brian Halsall, Cleves, OH (US);
Amadeo J Pesce, Cincinnati, OH (US);
University of Cincinnati, Cincinnati, OH (US);
Abstract
The concentration of NADH or NADPH in a test solution is determined by adding a redox coupling agent, preferably 2,6 dichloroindophenol DCIP, to the test solution. The coupling agent reacts with the NADH or NADPH to form an electroactive coupling agent (DCIPH.sub.2) which is then detected electrochemically at a lower voltage than would be required to detect the NADH or NADPH. This can be used to detect NADH or NADPH formed by any well known enzymatic or immunoassay method which produces NADH as a detectable product. This has particular application to biological fluids such as whole blood which does not require any treatment of the test sample prior to electrochemical analysis. In particular, red blood cells do not have to be removed from whole blood samples to provide reliable data.