The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 17, 1993
Filed:
May. 24, 1991
Pramod K Gupta, Federal Way, WA (US);
Gerald S Pullman, Renton, WA (US);
Other;
Abstract
The invention is a method for reproducing coniferous trees by somatic embryo-genesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage proembryos. These are multiplied in a second culture having reduced growth hormones. The early stage embryos may then be placed in or on a late stage proembryo development culture with a significantly higher osmotic potential than the previous stage or stages in order to develop very robust late stage proembryos having at least 100 cells. Culturing from this point continues in a cotyledonary embryo development medium having a raised osmotic potential. Exogenous abscisic acid is now required at this stage. The concentration of abscisic acid is decreased over time to low levels either by the use of an adsorbent such as activated charcoal or by stepwise subcultures in which the later cultures have lower ABA concentration. After a period of several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth.