The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 20, 1993

Filed:

Feb. 03, 1992
Applicant:
Inventors:

Jinsei Miyazaki, Osaka, JP;

Noboru Motoyama, Osaka, JP;

Tadayasu Mitsumata, Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ;
U.S. Cl.
CPC ...
436537 ; 436546 ; 436816 ;
Abstract

A fluorescence immunoassay method is described in which the presence or absence of antigen is measured within one minute by measuring a variation in fluorescence emitted from an antibody. The method comprises reaction between a pseudo-antigen obtained by chemical combination of an antigen and a fluorescent quencher and an antibody by which fluorescence to be emitted from the antibody is quenched by the action of the quencher combined. When an analyte is added to a solution of the combination, the pseudo-antigen is replaced by an antigen if the analyte contains the antigen, so that the fluorescence increases in intensity. This intensity is measured and compared with the initially measured intensity to detect the presence of the antigen. The method does not make use of the action of antigen on antibody with respect to the fluorescence of the antibody and is applicable to all antigens.


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