The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 20, 1993

Filed:

Aug. 09, 1989
Applicant:
Inventors:

Renee Kosslak, Madison, WI (US);

Roger Bookland, Madison, WI (US);

Edward R Appelbaum, Madison, WI (US);

Assignee:

Lubrizol Genetics, Inc., Wickliffe, OH (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01N / ; A01N / ; C12R / ; C12N / ; C05F / ; A01C / ;
U.S. Cl.
CPC ...
424 / ; 4352522 ; 435878 ; 71-7 ; 47 576 ; 47DI / ; 47DI / ; 424 / ; 504117 ;
Abstract

The chemical compounds that induce expression of nodulation genes of Bradyrhizobium japonicum strains have been identified. The isoflavones daidzein and genistein were identified as soybean exudate components that induce nod-genes. Other isoflavones, including 7-hydroxyisoflavone, 5,7-dihydroxyisoflavone, biochanin A, formononetin and prunetin, were also found to act as inducers. In addition, several flavonoid compounds including 4',7-dihydroxyflavone, apigenin, kaempferol and coumestrol, were also found to act as inducers. Nodulation inducing compositions and a method for selectively activating the expression of a gene under the control of a soybean exudate inducible promoter in B. japonicum in response to these inducer molecules are described. In addition, soybean inoculating compositions containing the nod-gene inducers and inoculating methods employing these compositions are described.


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