The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 13, 1993

Filed:

Oct. 23, 1992
Applicant:
Inventors:

Paul E Holmes, Hamden, CT (US);

Jon A Kornacki, Wallingford, CT (US);

Assignee:

Olin Corporation, Cheshire, CT (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ;
U.S. Cl.
CPC ...
435198 ; 435271 ;
Abstract

A novel lipase from a newly-discovered strain of Pseudomonas alcaligenes microorganism having (i) an optimum pH for activity of about 10.+-.0.5; (ii) an optimum temperature for activity of about 45.degree. to 55.degree. C.; (iii) an optimum pH for stability of about 7.0.+-.0.5; (iv) a molecular weight as measured by SDS-PAGE of about 3.0.times.10.sup.4 ; and (v) chemical stability for at least a 60 day mean half-life in the presence of a 10 percent solution of polyoxyethylene (23) lauryl ether in 25 millimolar aqueous calcium chloride. Also claimed is a biologically pure culture of the microorganism, and a method for the production of the lipase. Also claimed is a lipase characterized by containing an N-terminal amino acid sequence which is Gly-Leu-Phe-Gly-Pro-Ser-Gly-Tyr-Thr-Lys-Thr-Lys-Tyr-Pro-Ile.


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