The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 15, 1993
Filed:
Mar. 31, 1992
Chris Brinegar, Boulder Creek, CA (US);
James Gilmore, San Jose, CA (US);
Michael Johnson, San Jose, CA (US);
Nigel Walker, Oakland, CA (US);
Other;
Abstract
Methods and apparatus for the introduction of foreign substances, such as recombinant DNA, into living cell interiors is disclosed. The desired substance to be introduced is dissolved or suspended in water or another suitable liquid to form a solution which is then nebulized and frozen to produce ice particles. These ice particles are accelerated toward and impact upon a target tissue where some of the particles impregnate at least some cells in the target tissue without killing the cells. Following impregnation, the ice particles melt, leaving behind the desired substance in the protoplasm of the bombarded cell.