The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 11, 1993
Filed:
Aug. 06, 1990
Applicant:
Inventors:
David H Gelfand, Oakland, CA (US);
Pamela M Holland, Oakland, CA (US);
Randall K Saiki, Richmond, CA (US);
Robert M Watson, Berkeley, CA (US);
Assignee:
Hoffman-La Roche Inc., Nutley, NJ (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435 18 ; 435 91 ; 435196 ; 435805 ; 436501 ; 436 63 ; 436815 ; 536 243 ; 935 17 ; 935 77 ; 935 78 ; 935 88 ;
Abstract
The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Published as:
CA2088683A1; WO9202638A1; AU8651091A; US5210015A; EP0543942A1; JPH06500021A; US5487972A; AU666837B2; US5804375A; JP2825976B2; EP0919565A2; CA2088683C; EP0919565A3; EP0543942B1; ATE198912T1; DE69132521D1; US6214979B1; ES2155058T3; DK0543942T3; DE69132521T2; EP1302549A2; EP0919565B1; ATE252110T1; DE69133329D1; EP1302549A3; DK0919565T3; ES2209033T3; DE69133329T2; US2005255499A1; EP0543942B2; US7141377B2; DK0543942T4; ES2155058T5; EP1302549B1; DE69132521T3; ATE365812T1; DE69133574D1; DK1302549T3; ES2289190T3; DE69133574T2; US2008171315A1; US7445900B2; EP0919565B2; DK0919565T4; ES2209033T5; DE69133329T3;