The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 06, 1993
Filed:
Jul. 02, 1990
Applicant:
Inventors:
Huimin Kong, Beverly, MA (US);
Ira Schildkraut, Hamilton, MA (US);
Assignee:
New England Biolabs, Inc., Beverly, MA (US);
Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
435199 ; 435832 ;
Abstract
The present invention provides a novel Type II restriction endonuclease obtainable from Bacillus coagulans. The endonuclease known as Bcg I, recognizes the following nucleotide sequence and has a cleavage point at both ends outside of its recognition sequence: ##STR1## to produce a 34 base pair fragment. Also described is a process for obtaining purified Bcg I from Bacillus coagulans, as well as processes for mapping chromosomal DNA and methods for reducing background in transformants with enzymes such as Bcg I.