Monoclonal antibody to human lymphotoxin and use thereof

Abstract

A monoclonal antibody reactive with a C-terminal half of human lymphotoxin, and fragments thereof; a hybridoma cell line producing a monoclonal antibody reactive with a C-terminal half of human lymphotoxin; a process for the production of a monoclonal antibody reactive with a C-terminal half of human lymphotoxin, comprising the steps of culturing the above-mentioned hybridoma in a medium to secrete the antibody, and recovering the antibody from the culture supernatant; a process for the production of a monoclonal antibody reactive with a C-terminal half of human lymphotoxin, comprising the steps of inoculating a hybridoma to a mammal, obtaining ascites from the mammal, and recovering the monoclonal antibody from the ascites; a process for the production of a hybridoma cell line producing a monoclonal antibody reactive with a C-terminal half of human lymphotoxin, comprising the step of immunizing a mammal with a conjugate of zinc and a purified recombinant human lymphotoxin, obtaining spleen cells from the immunized mammal, fusing the spleen cells with myeloma cells, and cloning a hybridoma producing the monoclonal antibody; an adsorbent for lymphotoxin comprising a solid carrier and the above-mentioned monoclonal antibody or fragment of the antibody, wherein the monoclonal antibody is bonded to the surface of the solid carrier; a process for the purification of lymphotoxin comprising the steps of placing a material containing lymphotoxin in contact with the above-mentioned adsorbent to adsorb the lymphotoxin to the adsorbent, and eluting the adsorbed lymphotoxin with an eluent; and an assay method or assay kit for lymphotoxin using the above-mentioned monoclonal antibody or fragment thereof.