The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 09, 1993

Filed:

Jun. 24, 1991
Applicant:
Inventors:

William E Keating, Durham, NC (US);

Jillian A Robson, Pittsboro, NC (US);

Assignee:

Becton Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12N / ; C12P / ; C07H / ;
U.S. Cl.
CPC ...
435-6 ; 435220 ; 4352531 ; 435270 ; 435 91 ; 536 231 ;
Abstract

The invention provides a rapid method for lysing Mycobacteria. In one embodiment is provided a method for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of the enzyme achromopeptidase. The method of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.


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